Fabrication and Demonstration of a 3D-printing/PDMS Integrated Microfluidic Device

Fabrication and Performance of a Photonic-Microfluidic Integrated Device

Fabrication and performance of a functional photonic-microfluidic flow cytometer is demonstrated. The devices are fabricated on a Pyrex substrate by photolithographically patterning the microchannels and optics in a SU-8 layer that is sealed via a poly(dimethylsiloxane) (PDMS) layer through a unique chemical bonding method. The resulting devices eliminate the free-space excitation optics throug...

Control and automation of multilayered integrated microfluidic device fabrication.

Integrated microfluidics is a sophisticated three-dimensional (multi layer) solution for high complexity serial or parallel processes. Fabrication of integrated microfluidic devices requires soft lithography and the stacking of thin-patterned PDMS layers. Precise layer alignment and bonding is crucial. There are no previously reported standards for alignment of the layers, which is mostly perfo...

Design and Fabrication of a Membrane Integrated Microfluidic Cell Culture Device Suitable for High-Resolution Imaging

Fabrication of Biomimetic 3d Liver Tissue Using Photodegradable Hydrogels and Perfusion Culture in a Microfluidic Device

This paper reports a novel technique to fabricate perfusable 3D liver tissue in a microfluidic device using photodegradable hydrogels prepared with gelatin. Also, we performed the perfusion culture of human hepatocellular carcinoma (HepG2) cells encapsulated in the patterned hydrogels.

Hot embossing for fabrication of a microfluidic 3D cell culture

Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microe...